ChBC-83P: Biochemical Engineering Laboratory
Course Objective:
The purpose is to impart fundamental knowledge with respect to the equipments and techniques essential for carrying out fermentation for generation and analysis of the data and finally development of the bioprocess.
Course Outcomes
Upon the completion of this course the students will be able to:
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CO1
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Acquire basic knowledge of various equipments used in biochemical engineering lab.
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CO2
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Fundamental understanding of techniques with respect to sterilization, preparation of solid and liquid media, culture growth and preservation. |
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CO3
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Basic understanding of estimation techniques for biomass, substrate and product.
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CO4
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Generation and analysis of data for design and development of bioprocess.
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List of Experiments
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S.No
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Experiment
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1
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Study the bioreactor, shaking incubator, spectrophotometer, HPLC, laminar flow chamber, autoclave, centrifuge. w.r.t. their construction, application, operation and working principle.
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2
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To prepare basic solid media as agar slants and agar plates.
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3
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Study of sterilization by application of a steam autoclave.
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4
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Quantitative estimation of glucose concentration by DNS colorimetric method or by phenol-sulfuric acid method.
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5
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Estimation of cell concentration.
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6
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Determination of volumetric mass-transfer co-efficient of O2 by static method.
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| 7 |
Determination of volumetric mass-transfer co-efficient of O2 by dynamic method.
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| 8 |
To study the kinetics of alcohol (ethyl alcohol) fermentation by using baker’s yeast (Saccharomyces cerevisae) in a batch bioreactor.
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List of Equipment
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S.No
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Equipment
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1
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Bioreactor
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2
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Shaking Incubator
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3
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Spectrophotometer
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4
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Laminar Flow Chamber (Bio-safety Cabinet)
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5
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Autoclave
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6
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Centrifuge
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| 7 |
Electronic Balance
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| 8 |
Oven |